UNITED STATES

                       SECURITIES AND EXCHANGE COMMISSION

                             Washington, D.C. 20549

                                    FORM 10-K

(Mark One)
(X)  Annual  Report  Pursuant  to  Section  13 or 15(d) of the  Securities
     Exchange Act of 1934. For the fiscal year ended December 31, 2000 or

( )  Transition Report Pursuant to Section 13 or 15(d) of the Securities
     Exchange Act of 1934.

             For the transition period from__________ to __________.

                         Commission file number 2-89283

                           IOWA FIRST BANCSHARES CORP.
             ------------------------------------------------------
             (Exact name of registrant as specified in its charter)

      An Iowa Corporation                                  42-1211285
-------------------------------                ---------------------------------
(State or other jurisdiction of                (I.R.S. Employer incorporation or
        organization)                                  Identification No.)

300 East Second Street, Muscatine, Iowa                      52761
----------------------------------------                   ----------
(Address of principal executive offices)                   (Zip Code)

Registrant's telephone number, including area code   (319) 263-4221
Securities registered pursuant to Section 12(b) of the Act:  None
Securities registered pursuant to Section 12(g) of the Act:  None

Indicate by check mark whether the registrant (1) has filed all reports required
to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during
the  preceding 12 months (or for such  shorter  period that the  registrant  was
required  to file  such  reports),  and  (2) has  been  subject  to such  filing
requirements for the past 90 days. [ X ]  Yes    [  ]  No

Indicate by check mark if disclosure of delinquent  filers  pursuant to Item 405
of Regulation  S-K is not contained  herein,  and will not be contained,  to the
best of registrant's  knowledge,  in definitive proxy or information  statements
incorporated by reference in Part III of this Form 10-K or any amendment to this
Form 10-K. (X)

The  aggregate  market  value of the voting stock held by  nonaffiliates  of the
registrant as of February 28, 2001,  was  $26,230,578.  As of February 28, 2001,
1,493,331 shares of the Registrant's common stock were outstanding.

Documents incorporated by reference:

Portions of the registrant's  2000 Annual Report are incorporated in Parts I and
II of this Form 10-K.  Portions of the registrant's  Proxy Statement dated March
12, 2001 are incorporated in Part III of this Form 10-K.

The Exhibit Index is located on page 11.



                           ANNUAL REPORT ON FORM 10-K

                                TABLE OF CONTENTS

                                     PART I

                                                                            Page
                                                                             No.
                                                                            ----

Item  1.  Business
Item  2.  Properties
Item  3.  Legal Proceedings
Item  4.  Submission of Matters to a Vote of Security Holders
Table I.  Executive Officers of the Registrant

                                     PART II

Item  5.  Market for the Registrant's Common Equity and Related
            Stockholder Matters
Item  6.  Selected Financial Data
Item  7.  Management's Discussion and Analysis of Financial Condition and
            Results of Operations
Item  8.  Financial Statements and Supplementary Data
Item  9.  Changes in and Disagreements with Accountants on Accounting and
            Financial Disclosure


                                    PART III

Item 10.  Directors and Executive Officers of the Registrant
Item 11.  Executive Compensation
Item 12.  Security Ownership of Certain Beneficial Owners and Management
Item 13.  Certain Relationships and Related Transactions


                                     PART IV

Item 14.  Exhibits, Financial Statement Schedules, and Reports on Form 8-K
          Signatures
          Index of Exhibits




                           ANNUAL REPORT ON FORM 10-K

                                     PART I

ITEM 1. BUSINESS.

Iowa  First  Bancshares  Corp.  (the  "Company"),  is  a  bank  holding  company
headquartered in Muscatine,  Iowa. The Company owns all the outstanding stock of
two national banks in Iowa,  First National Bank of Muscatine and First National
Bank in Fairfield.

On a full-time  equivalent  basis,  year-end  employment for the Company and its
subsidiary banks totaled 123 employees.

First  National  Bank of Muscatine  has a total of five  locations in Muscatine,
Iowa. The First National Bank in Fairfield has two locations in Fairfield, Iowa.
Each bank is engaged in the general  commercial  banking  business  and provides
full service  banking to  individuals  and  businesses,  including  checking and
savings  accounts,  commercial  loans,  consumer loans,  real estate loans, safe
deposit  facilities,  transmitting  of funds,  trust  services,  and such  other
banking services as are usual and customary for commercial banks.

The commercial banking business is highly competitive.  Subsidiary banks compete
with other  commercial  banks and with other financial  institutions,  including
savings banks,  mortgage banking  companies,  credit unions and mutual funds. In
recent years,  competition also has increased from  institutions not necessarily
subject to all the same  regulatory  restrictions  or  requirements as banks and
bank holding companies.

The operations of the Company and its subsidiary banks are affected by state and
federal legislative  changes and by policies of various regulatory  authorities.
The Company is a registered  bank holding company under the Bank Holding Company
Act of 1956 (the "Act") and is subject to the supervision of, and regulation by,
the Board of Governors of the Federal  Reserve System (the  "Board").  Under the
Act, a bank  holding  company  may engage in banking,  managing  or  controlling
banks,  furnishing or performing services for banks it controls,  and conducting
activities that the Board has determined to be closely related to banking.

National  banks are  subject to the  supervision  of, and are  examined  by, the
Office of the Comptroller of the Currency.  Both subsidiary banks of the Company
are members of the  Federal  Deposit  Insurance  Corporation,  and as such,  are
subject to examination thereby. In practice, the primary federal regulator makes
regular examinations of each subsidiary bank subject to its regulatory review or
participates in joint examinations with other federal regulators.  Areas subject
to regulation by these  authorities  include capital  levels,  the allowance for
possible  loan losses,  investments,  loans,  mergers,  issuance of  securities,
payment of  dividends,  establishment  of  branches,  and many other  aspects of
operations.

Statistical  information  called for by this Item is contained in the  Company's
2000 Annual Report to Shareholders  which is incorporated by reference (pages 40
- 51 of this Form 10-K).

ITEM 2. PROPERTIES.

Since the Company commenced  business,  its principal  executive office has been
located at 300 East  Second  Street,  Muscatine,  Iowa,  which is the  principal
office of First National Bank of Muscatine, a national banking association and a
wholly owned subsidiary of the Company.

First National Bank of Muscatine  conducts its operations  from five  facilities
located in Muscatine.  The main bank is located at 300 East Second Street and is
a modern brick and steel  building  completed in 1979  containing  36,000 square
feet of floor space on three  floors.  The bank owns both the  building  and the
underlying real estate. All  administrative  functions of the bank are conducted
at its main offices.  Portions of the building are leased to commercial tenants.
During  1997,  a branch was  opened  inside  the then new  Wal-Mart  Supercenter
located on highway 61 at  Muscatine.  This branch and the  Wal-Mart  Supercenter
were the first of their kind in Iowa. The bank operates this branch under a five
year  lease  agreement  with  Wal-Mart,  with two  five  year  renewal  options.
Additionally,  another new branch facility, which includes drive-through banking
services  and is  located  across  the  alley  from the main  Muscatine  banking
headquarters, was completed in the fall of 1997. This branch replaced a previous
downtown branch. The bank owns this facility and the underlying real estate.



Two locations,  in addition to the new Wal-Mart branch, provide banking services
outside  the  Muscatine  downtown  area.  The  office at the  Muscatine  Mall is
approximately  two miles northeast of the main bank. The facility contains 2,304
square feet of floor  space in a  one-story  concrete  and steel  building.  The
facility offers a walk-in lobby and night  depository.  The three-lane  drive-up
facility of this branch is located  approximately 500 feet west of the branch at
the parking lot of the mall. The building,  drive-up  facilities and real estate
are leased. The terms of the lease provide for monthly payments of $2,304.  This
lease  has  expired.  Management  is  negotiating  with  the new  owners  of the
Muscatine Mall for construction of a freestanding building on Mall property with
a long-term lease arrangement with First National Bank of Muscatine.

The  bank's  southside  office  at 608  Grandview  Avenue is  located  two miles
southwest of the main bank. The office contains 3,600 square feet of floor space
and is located in a one-story steel frame, concrete block building. The facility
offers a walk-in lobby and three  drive-up  lanes.  The building and  underlying
real  estate  are owned by the bank.  Portions  of the  building  are  leased to
commercial tenants.

First National Bank in Fairfield conducts its operations from a modern brick and
steel building  completed in 1968 containing 8,200 square feet of floor space on
two floors.  The bank owns both the  building  and the  underlying  real estate.
Portions of the building are leased to commercial tenants. A three-lane drive-up
facility  is  located  at the main  bank.  In the  spring of 1997,  a new branch
facility was opened at Fairfield, Iowa. The building, which is located in a high
traffic  area in front of the  local  Wal-Mart  store on  highway  34,  contains
several  private  offices for lending staff and management as well as teller and
deposit services, including several drive-through lanes.

The Company's  facilities are well maintained and are suitable for the Company's
business operations.

ITEM 3.   LEGAL PROCEEDINGS.
----------------------------

The Company has no pending legal proceedings which are material.

ITEM 4. SUBMISSION OF MATTERS TO A VOTE OF SECURITY HOLDERS.

None.



                                 PART I, TABLE I

                      EXECUTIVE OFFICERS OF THE REGISTRANT

                             Family                               Position      Business Experience
Name                 Age   Relationship     Position             Held Since     During Past Five Years
------------------------------------------------------------------------------------------------------------------------------------
                                                                 
George A. Shepley     78      None       Chairman of the Board      1983        Chairman of the Board, 1983 to
                                         Director                   1983        Present, Chief Executive Officer,
                                                                                1983 to 2001, President, 1989 to
                                                                                1996, Iowa First Bancshares Corp.;
                                                                                Chairman of the Board, 1987 to present, President,
                                                                                1963 to 1989, First National Bank of Muscatine;
                                                                                Chairman of the Board, 1986 to
                                                                                present, First National Bank in
                                                                                Fairfield.

Kim K. Bartling       43      None       Executive Vice President   1996        Executive Vice President,
                                         Chief Operating Officer    1996        Chief Operating Officer
                                         Treasurer                  1988        and Treasurer of the
                                         Director                   1994        Company, December 1996 to
                                                                                present;  Senior Vice President,
                                                                                Chief Financial Officer and Treasurer
                                                                                of the Company, 1988 to 1996; Director of
                                                                                the Company since 1994;  Director of First
                                                                                National Bank of Muscatine, 1989 to present;
                                                                                Executive Vice President and
                                                                                Chief Financial Officer, First
                                                                                National Bank of Muscatine, 1996 to
                                                                                present; Senior Vice President/Chief
                                                                                Financial Officer, First National
                                                                                Bank of Muscatine, 1987 to
                                                                                1996; Director First National
                                                                                Bank in Fairfield, 1990 to present.

D. Scott Ingstad      50      None       Vice Chairman of                       Vice Chairman of the Board,
                                         the Board                  1999        October 1999 to present, Chief
                                         Chief Executive                        Executive Officer, January 2001 to
                                         Officer                    2001        present, Director, 1990 to present,
                                         President                  1996        President, 1996 to present, of the
                                         Director                   1990        Company; Director, President and  CEO,
                                                                                1990 to present, Vice Chairman of the
                                                                                Board, 1999 to present, First National
                                                                                Bank Of Muscatine.

Patricia R.           53      None       Secretary                1986          Corporate Secretary of
Thirtyacre                                                                      the Company, 1986 to present.


                                     PART II

ITEM 5. MARKET FOR THE REGISTRANT'S COMMON EQUITY AND RELATED
        STOCKHOLDER MATTERS

The  brokerage  firms of Howe Barnes  Investments,  Inc.  and Sandler  O'Neill &
Partners, L.P. make a market for the Company's common stock.

High and low common stock prices and dividends for the last two years were:

2000 by                                                               Dividend
Quarters                              High             Low            Per Share
--------------------------------------------------------------------------------

First .....................        $   24.00         $   22.13         $   0.21
Second ....................            23.00             22.00             0.21
Third .....................            23.00             18.00             0.21
Fourth ....................            23.00             18.50             0.21

Total Dividend
Paid                                                                      $0.84

1999 by                                                               Dividend
Quarters                              High            Low             Per Share
--------------------------------------------------------------------------------
First .....................        $   32.50         $   31.00         $   0.21
Second ....................            32.50             29.50             0.21
Third .....................            29.50             25.50             0.21
Fourth ....................            27.50             23.88             0.21

Total Dividend
Paid ......................                                           $    0.84



The above  quotations were furnished by the brokerage firms that serve as market
makers for the Company's stock. The quotations  represent prices between dealers
and do not include retail markup, markdown, or commissions.

Future dividends are dependent on future earnings,  regulatory restrictions (see
Management's  Discussion  and  Analysis of  Financial  Condition  and Results of
Operations  on  pages 40 - 51 of this  Form  10-K;  and Note 9 to the  Company's
Consolidated  Financial  Statements  in the  Company's  2000  Annual  Report  to
Shareholders  which is  incorporated  by  reference,  pages 30 - 32 of this Form
10-K), capital requirements, and the Company's financial condition.

As of February 28, 2001, the Company had  approximately  400 shareholders of its
outstanding  class of common stock.  The Iowa First  Bancshares  Corp.  Employee
Stock Ownership Plan with 401(k) Provisions is considered one shareholder as all
shares owned by this plan are voted by the trustees of said plan unless the vote
in  question  encompasses  approval  or  disapproval  of any  corporate  merger,
consolidation, dissolution, or similar transaction.

ITEM 6. SELECTED FINANCIAL DATA.

The  information  called for by this Item is  contained  in the  Company's  2000
Annual Report to  Shareholders  which is  incorporated  by reference (page 39 of
this Form 10-K).

ITEM 7. MANAGEMENT'S  DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS
          OF OPERATIONS.

The  information  called for by this Item is  contained  in the  Company's  2000
Annual Report to Shareholders  which is incorporated by reference (pages 40 - 51
of this Form 10-K).

ITEM 8. FINANCIAL STATEMENTS AND SUPPLEMENTARY DATA.

The  information  called for by this Item is  contained  in the  Company's  2000
Annual Report to Shareholders  which is incorporated by reference (pages 18 - 39
of this Form 10-K).

ITEM  9.  CHANGES  IN AND  DISAGREEMENTS  WITH  ACCOUNTANTS  ON  ACCOUNTING  AND
          FINANCIAL DISCLOSURE.

None.

                                    PART III

ITEM 10. DIRECTORS AND EXECUTIVE OFFICERS OF THE REGISTRANT

The information called for by this Item is contained in the Company's 2000 Proxy
Statement which is incorporated by reference (page 55 of this Form 10-K).

Director Compensation

The annual retainer that each outside  Director of the Company  received in 2000
was $5,500.  The Company paid $125 for attendance at each committee  meeting and
special Board of Directors  meeting.  During 2000,  each Director of the Company
served  as  Director  and  member  of  committees  for  subsidiary   boards  and
committees,  with the  exception of Mr.  Carver who served only as a Director of
the Company.  The annual retainer fee paid to each outside  subsidiary  Director
was  $4,200,  plus  $125 to $250  for  attendance  at  each  committee  meeting.
Executive  officers who also serve on the Board of Directors do not receive such
retainer or committee fees.  Additional  information  called for by this Item is
contained  in the  Company's  2000  Proxy  Statement  which is  incorporated  by
reference (page 59 of this Form 10-K).

ITEM 11. EXECUTIVE COMPENSATION

The information called for by this Item is contained in the Company's 2000 Proxy
Statement which is incorporated by reference (pages 57 - 59 of this Form 10-K).



ITEM 12. SECURITY OWNERHSIP OF CERTAIN BENEFICIAL OWNERS AND MANAGEMENT.

The information called for by this Item is contained in the Company's 2000 Proxy
Statement  which is  incorporated  by  reference  (pages  54 and 55 of this Form
10-K).

ITEM 13. CERTAIN RELATIONSHIPS AND RELATED TRANSACTIONS.

Officers  and  Directors of the Company and its  subsidiaries  have had, and may
have in the future,  banking  transactions in the ordinary course of business of
the Company's subsidiaries.  All such transactions are on substantially the same
terms, including interest rates on loans and collateral,  as those prevailing at
the time for comparable  transactions with others,  and involve no more than the
normal risk of collectibility.


                                     PART IV

 ITEM 14. EXHIBITS, FINANCIAL STATEMENT SCHEDULES, AND REPORTS ON FORM 8-K.

         (a) Documents Filed with This Report:

             (1) Financial  Statements.  The  following  consolidated  financial
                 statements of the Company and its subsidiaries are incorporated
                 by reference from the 2000 Annual Report to Shareholders of the
                 Company:

                                                                            Page

             Consolidated balance sheets -- dated December 31, 2000 and 1999.

             Consolidated statements of income -- years ended December 31,
             2000, 1999, and 1998.

             Consolidated statements of changes in stockholders' equity --
             years ended December 31, 2000, 1999, and 1998.

             Consolidated statements of cash flows - years ended
             December 31, 2000, 1999, and 1998.

             Notes to consolidated financial statements.

             Opinion of independent accountants.

         (2) Financial  Statement  Schedules.  All schedules are omitted because
             they are not applicable,  are not required, or because the required
             information  is included in the  financial  statements or the notes
             thereto.

             (b) Reports on Form 8-K.

                 No reports on Form 8-K have been filed  during the last
                 quarter of the period covered by this report.

             (c) Exhibits.  The following exhibits are attached pursuant to Item
                 601 of Regulation S-K:

                  (10c)  Executive Deferred Comp Agreements
                         1)  Kim Bartling
                         2)  Timothy Nelson
                         3)  Dean Holst
                         4)  Scott Ingstad
                  (10d)  Director Deferred Fee Agreement
                  (13)   Registrant's 2000 Annual Report to Shareholders
                  (20)   Registrant's Proxy Statement dated March 12, 2001
                  (24)   Power of Attorney

See Exhibit Index on page 11 hereof for a complete list of management  contracts
and  arrangements  required  by  this  item  and all  other  Exhibits  filed  or
incorporated by reference as a part of this report.




                                   SIGNATURES

Pursuant to the  requirements of Section 13 or 15(d) of the Securities  Exchange
Act of 1934,  the  registrant  has duly  caused  this report to be signed on its
behalf by the undersigned, thereunto duly authorized.

                                                 IOWA FIRST BANCSHARES CORP.

Date:  March 9, 2001                             /s/  George A. Shepley
       -------------                             -------------------------------
                                                 George A. Shepley
                                                 Chairman of the Board

Date:  March 9, 2001                             /s/  Kim K. Bartling
       -------------                             -------------------------------
                                                 Kim K. Bartling, Executive Vice
                                                 President, Chief Operating
                                                 Officer, Treasurer and Director
                                                 (Principal Financial and
                                                 Accounting Officer)

We, the undersigned  directors of Iowa First Bancshares  Corp.  hereby severally
constitute  George A. Shepley and Kim Bartling,  and each of them,  our true and
lawful  attorneys  with full power to them, and each of them, to sign for us and
in our name, the capacities  indicated  below, the Annual Report on Form 10-K of
Iowa First  Bancshares  Corp. for the fiscal year ended December 31, 2000, to be
filed herewith and any  amendments to said Annual  Report,  and generally do all
such things in our name and behalf in our capacities as directors to enable Iowa
First Bancshares Corp. to comply with the provisions of the Securities  Exchange
Act of 1934 as amended,  and all  requirements  of the  Securities  and Exchange
Commission, hereby ratifying and confirming our signatures as they may be signed
by our said attorneys, or either of them, to said Annual Report on Form 10-K and
any and all amendments thereto.

Pursuant to the requirements of the Securities Exchange Act of 1934, this report
has been signed below by the following  persons on behalf of the  registrant and
in the capacities and on the dates indicated.

        Signature                            Title             Date

/s/ Roy J. Carver, Jr.                      Director           February 15, 2001
----------------------                                         -----------------
Roy J. Carver, Jr.

/s/ Larry L. Emmert                         Director           February 15, 2001
-------------------                                            -----------------
Larry L. Emmert

/s/ Craig R. Foss                           Director           February 15, 2001
-----------------                                              -----------------
Craig R. Foss

/s/ Donald R. Heckman                       Director           February 15, 2001
---------------------                                          -----------------
Donald R. Heckman

/s/ Dean H. Holst                           Director           February 15, 2001
-----------------                                              -----------------
Dean H. Holst

/s/ David R. Housley                        Director           February 15, 2001
--------------------                                           -----------------
David R. Housley

/s/ D. Scott Ingstad                        Director           February 15, 2001
--------------------                                           -----------------
D. Scott Ingstad

/s/ Victor G. McAvoy                        Director           February 15, 2001
--------------------                                           -----------------
Victor G. McAvoy

/s/ John "Jay" S. McKee                     Director           February 15, 2001
-----------------------                                        -----------------
John "Jay" S. McKee

/s/ Beverly J. White                        Director           February 15, 2001
--------------------                                           -----------------
Beverly J. White




                       ITEM 14 (a) (3) - INDEX OF EXHIBITS

         Exhibit                                   Page
                                                
(3)   Articles of Incorporation, as amended        Incorporated by reference to Exhibit
                                                   (3) to the registrant's Annual Report
                                                   on Form 10-K for the fiscal year ended
                                                   December 31, 1996.

(10a) Employment Agreement                         Incorporated by reference to Exhibit
                                                   (10a) to the registrant's Annual
                                                   Report on Form 10-K for the fiscal
                                                   year ended December 31, 1995.

(10b) Change in Control Employment Agreement       Incorporated by reference to Exhibit
                                                   (10b) to the registrant's Annual
                                                   Report on Form 10-K for the fiscal
                                                   year ended December 31, 1995.

(10c) Executive Deferred Compensation Agreements

(10d) Director Deferred Fee Agreement

(13)  Registrant's 2000 Annual Report to Shareholders

(20)  Registrant's Proxy Statement Dated March 12, 2001

(21)  Subsidiaries of Registrant                   Incorporated by reference to Exhibit 21
                                                   to the registrant's Annual Report on
                                                   Form 10-K for the fiscal year ended
                                                   December 31, 1995.

(24)  Power of Attorney